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Fig. 5 | Phytopathology Research

Fig. 5

From: The small GTPase BcSec4 is involved in conidiophore development, membrane integrity, and autophagy in Botrytis cinerea

Fig. 5

BcSEC4 is required for full virulence of B. cinerea. Phenotypes of tomato (a) and mung bean leaves (b) after drop-inoculated with conidial suspensions (1 × 105 conidia/mL) of B05.10 and the ΔBcSEC4 mutant for 3 days. Column diagram showing lesion size on leaves of tomato (c) and mung bean (d) corresponding to a and b. e Penetration assay performed on onion epidermis and observed under a microscope at 12 hpi. Red arrows: appressoria; Black arrows: conidia; Yellow arrows: invasive hyphae. f Relative penetration rate on onion epidermis at 12 hpi. At least 50 infection hyphae were counted for each strain. g The lengths of invasive hyphae measured at 12 hpi. h The expansion of B05.10 and the ΔBcSEC4 mutant in onion epidermal cells (yellow arrows) observed under a microscope at 36 hpi. All data are represented as means ± standard deviations (SDs) from three independent experiments. Different letters indicate statistically significant differences (P < 0.01)

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