Fig. 5From: The small GTPase BcSec4 is involved in conidiophore development, membrane integrity, and autophagy in Botrytis cinereaBcSEC4 is required for full virulence of B. cinerea. Phenotypes of tomato (a) and mung bean leaves (b) after drop-inoculated with conidial suspensions (1 × 105 conidia/mL) of B05.10 and the ΔBcSEC4 mutant for 3 days. Column diagram showing lesion size on leaves of tomato (c) and mung bean (d) corresponding to a and b. e Penetration assay performed on onion epidermis and observed under a microscope at 12 hpi. Red arrows: appressoria; Black arrows: conidia; Yellow arrows: invasive hyphae. f Relative penetration rate on onion epidermis at 12 hpi. At least 50 infection hyphae were counted for each strain. g The lengths of invasive hyphae measured at 12 hpi. h The expansion of B05.10 and the ΔBcSEC4 mutant in onion epidermal cells (yellow arrows) observed under a microscope at 36 hpi. All data are represented as means ± standard deviations (SDs) from three independent experiments. Different letters indicate statistically significant differences (P < 0.01)Back to article page