Fig. 6

BcSec4 is required for membrane integrity of B. cinerea. a Plasma membrane integrity of B05.10 and the ΔBcSEC4 mutant assessed by staining with the small molecule propidium iodide (PI). b ROS accumulation assessed by DCFH-DA staining. c Fluorescence accumulation in the supernatant of conidial suspension. d Transmission electron microscopy observation of the plasma membranes of B05.10 and the ΔBcSEC4 mutant cultured in YEPD liquid medium for 12 h. Arrowhead indicates a site of cell membrane damage. e The conductivity of B05.10 and the ΔBcSEC4 mutant measured after inoculation for 24 h. All data are represented as means ± standard deviations (SDs) from three independent experiments. Different letters indicate statistically significant differences (P < 0.01)