Fig. 4

Gene trees of AsCEP19 and AsCEP20 and species tree of the genus Alternaria. The best amino acid substitute models were determined using ProtTest. Maximum-likelihood trees were constructed using RAxML. a Phylogenetic trees constructed according to amino acid sequences of AsCEP19, AsCEP20 and their homologous proteins. The numbers shown next to the branches indicate the percentage of bootstrap support values (1000 replicates). The branch lengths indicate the evolutionary distance as the number of base substitutions per site. The rectangular boxes represent the changes in the gene expression during fungal infection. NA, data not available; b Phylogenetic tree constructed according to the alignments of 1,216 single-cpoy protein-coding genes from 37 fungal genome datasets. The 1,216 orthogroups were aligned using MAFFT, and these multiple alignments were trimmed and concatenated into a super-matrix