Fig. 8

Variation in virulence between the gene pair (AsCEP19 and AsCEP20)-knockout mutant and wild-type of A. solani on potato leaves. a Detached potato leaves inoculated with conidial suspensions of the wild-type strain (left) and the ΔAsCEP19 + AsCEP20 mutant (right). The sizes of necrotic lesions were measured at 5 dpi. b The average size of necrotic lesions caused by the ΔAsCEP19 + AsCEP20 mutant and the wild-type strain. Error bars indicate the standard deviation of the mean, and asterisks indicate statistical significance determined by paired t-test (t = 16.16, p-value = 2.5e−11, df = 16, n = 17), with three (***) P < 0.001