Skip to main content
Fig. 5 | Phytopathology Research

Fig. 5

From: GmPAO-mediated polyamine catabolism enhances soybean Phytophthora resistance without growth penalty

Fig. 5

GmPAO significantly enhances resistance against P. sojae in the transgenic soybean lines. The soybean lines were the same as in Fig. 1. Two P. sojae isolates (P7076 and PS14) that are virulent on WT were used. a, b Representative disease phenotypes at 36 hpi (a) and 60 hpi (b). Hypocotyls or unifoliate leaves were inoculated with P. sojae P7076. Scale bars, 1 cm. c Representative disease phenotypes of seedlings at 12 dpi. The seedlings were inoculated with P. sojae P7076 (5 × 104 zoospores/mL) using the root-dipping inoculation method. Scale bar, 5 cm. d, e Comparisons of the hypocotyl lesion lengths and leaf lesion diameters caused by P. sojae P7076 or P. sojae PS14. Error bar represents mean ± SD, n ≥ 12, n represents sample number. f, g Quantitation of resistance levels via assessment of P. sojae biomass accumulations in inoculated hypocotyls and leaves by genomic qPCR. Error bar represents mean ± SD, n = 3, n represents sample number. h Seedling disease ratings in the transgenic lines over time. The disease symptoms were recorded at 4, 8, and 12 dpi. Disease ratings are shown with different colors (n ≥ 12, n represents sample number). All the experiments were performed three times (biological replicates) with similar results. The data in d–h were analyzed by Shapiro–Wilk test to determine the Normality and Lognormality Tests across groups, and then analyzed by one-way ANOVA with post-hoc Dunnett's multiple comparisons test for groups that had passed the normality test (ns, no significant difference; **P < 0.01). The exact n, SD values, and P values are shown in the data

Back to article page