Fig. 6
From: GmPAO-mediated polyamine catabolism enhances soybean Phytophthora resistance without growth penalty
GmPAO enhances resistance to P. capsici and P. parasitica infections in N. benthamiana. a, e Lesions caused by P. capsici (a) or P. parasitica (e) on N. benthamiana leaves expressing GFP or GmPAO genes. GFP was used as a negative control. Photos were taken at 36 hpi. Scale bars, 1 cm. b, f Comparisons of the lesion diameter caused by P. capsici (b) or P. parasitica (f). Error bar represents mean ± SD, n ≥ 12, n represents sample number. c, g Relative pathogen biomass quantified by genomic qPCR. Error bar represents mean ± SD, n = 3, n represents sample number. d, h Representative immunoblot showing the levels of GFP and GmPAO after transient expression in N. benthamiana leaves. Proteins were stained with Ponceau S to confirm equal loading. All the experiments were performed three times (biological replicates) with similar results. The data in b, c, f, and g were analyzed by Shapiro–Wilk test to determine the Normality and Lognormality Tests across groups, and then analyzed by unpaired t test for groups that had passed the normality test (**P < 0.01). The exact n, SD values, and P values are shown in the data