Fig. 7

Agronomic traits of GmPAO overexpression soybean lines. The soybean lines were the same as in Fig. 1. a Growth phenotypes (upper) of soybean plants grown in growth chambers for two months. Scale bar, 20 cm. Pod (middle) and Seed (lower) morphology after harvest. Scale bars, 1 cm. b Statistical analysis of 100-seed weight of the transgenic soybean plants. The seeds were randomly chosen and weighed. Error bar represents mean ± SD, n = 20, n represents sample number. c Germination rate of the transgenic soybean plants. The number of germinated seeds was recorded after gemination for 8 days. Error bar represents mean ± SD, n = 3 experiments with 40 seeds. All the experiments were performed three times (biological replicates) with similar results. The data in b and c were analyzed by Shapiro–Wilk test to determine the Normality and Lognormality Tests across groups, and then analyzed by one-way ANOVA with post-hoc Dunnett's multiple comparisons test for groups that had passed the normality test (ns, no significant difference). The exact n, SD values, and P values are shown in the data