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Fig. 3 | Phytopathology Research

Fig. 3

From: Detection and characterization of rice orange leaf phytoplasma infection in rice and Recilia dorsalis

Fig. 3

Electron micrographs showing the entry of ROLPs into the intestinal epithelium of R. dorsalis. a Intestinal epithelium, showing microvilli on the lumen side and basal lamina covered with muscle fibers and serosal barrier on the hemocoel side. b ROLP distribution in the gut lumen. ROLP-infected intestines were incubated with Imp-specific antibody and then with 15-nm gold particle-conjugated goat antibody against rabbit IgG as secondary antibody. c–j Process for entry of ROLPs from the gut lumen into epithelial cells by successively passing through the perimicrovillar membrane (c–e), microvilli (f, g), and apical plasmalemma (h–j). Arrows indicate ROLPs. AP, apical plasmalemma; EC, epithelial cell; GL, gut lumen; MV, microvilli; PM, perimicrovillar membrane; SB, serosal barrier. Black arrows indicate gold particles. Red arrows indicate ROLPs. Scale bars, 2 μm (a) and 200 nm (b–j)

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Phytopathology Research

ISSN: 2524-4167