Fig. 4

Electron micrographs showing the release of ROLPs from intestinal epithelium to salivary glands via the hemocoel of R. dorsalis. a, b Attachment of ROLPs to the basal lamina of the midgut. b is the enlarged image of boxed area in a. c Distribution of ROLPs in the space between the basal lamina and visceral muscle of the midgut. d Distribution of ROLPs in the space between the visceral muscle and the serosal barrier of the midgut. e–h Process for movement of ROLPs through the serosal barrier of the midgut. i, j Attachment of ROLPs to the basal lamina of salivary glands. j is the enlarged image of boxed area in i. k–n Process for movement of ROLPs through the basal lamina from the hemocoel into the salivary cell. l is the enlarged image of boxed area in k. BL, basal lamina; EC, epithelial cell; He, hemocoel; SB, serosal barrier; SC, salivary cytoplasm; VM, visceral muscle. Red arrows indicate ROLPs. Scale bars, 200 nm