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Fig. 6 | Phytopathology Research

Fig. 6

From: Detection and characterization of rice orange leaf phytoplasma infection in rice and Recilia dorsalis

Fig. 6

Electron micrographs showing the multiplication of ROLPs in vector midgut epithelium to produce offspring. a, b One ROLP mother cell was reproducing one daughter cell (arrows). b is the enlarged image of boxed area in a. c More offspring were reproduced to form the grapelike clusters (arrows). d Abundant ROLP offspring were reproduced throughout the epithelial cytoplasm. e–g ROLPs of various sizes reacted with Imp-specific antibody. h–m The process for the budding strategy used by ROLPs to produce offspring. The cellular protrusion of a ROLP cell (h, i) was gradually enlarged to initiate the formation of a bud (j, k), and then offspring was produced by fission at their junction (l, m). n Proposed model of the budding reproduction strategy. ROLP-infected intestines shown in e–m were incubated with Imp-specific antibody and then with 15-nm gold particle-conjugated goat antibody against rabbit IgG as secondary antibody. Arrows indicate gold particles. BL, basal lamina; CP, cellular protrusion; EC, epithelial cell; He, hemocoel; MV, microvilli; SB, serosal barrier; VM, visceral muscle. Scale bars, 500 nm (a–d) and 200 nm (e–m)

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