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Fig. 4 | Phytopathology Research

Fig. 4

From: CRISPR/Cas9-targeted mutagenesis of a representative member of a novel PR10/Bet v1-like protein subfamily significantly reduces rice plant height and defense against Meloidogyne graminicola

Fig. 4

CRISPR/Cas9-targeted mutagenesis of OsBet v1. a Two target sites of CRISPR/Cas9 in exons. b Diagram of the binary vector pYLC-OsBet v1. c The mutation sequences of OsBet v1 knockout rice lines. The number of base deletion and/or insertion is shown by the mark of minus (−) and plus (+) followed by a number in brackets, respectively. Nucleotides in blue represent PAM sequences. Red letters and red hyphens denote insertions and deletions, respectively. d Sequence peaks of the wild-type (WT) and mutation types at target sites. The red arrowheads indicate the location of mutations. e Alignments of the deduced amino acid sequence of OsBet v1 from OsBet v1 knockout rice lines and WT. Asterisks indicate the termination of translation

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Phytopathology Research

ISSN: 2524-4167