Table 1 Detection of the putative off-target effect in potential off-target sites of the rice mutants
gRNA | Putative off-target gene | Off-target sequence | No. of mismatches* | Mutation |
|---|---|---|---|---|
Target1 | OS11G0162700 | GCTCAGTCACGAAATGCTCTTGG | 4 | Not detected |
OS01G0748200 | GTTCAGCCCCGGGATCCTGTCGG | 4 | Not detected | |
OS03G0828800 | GATGAGCCCCCAATTGCTGGTGG | 4 | Not detected | |
Target2 | OS04G0409100 | GCTGATCACGACATAGCCGAAAG | 3 | Not detected |
OS01G0717700 | CCTCATCACGTCATAGCCGAAGG | 4 | Not detected | |
OS06G0137700 | GCTGTCTTCTTCATAGCCGGCGG | 4 | Not detected | |
OS12G0570400 | GCTGTCTTCTTCATAGCCGGCGG | 4 | Not detected | |
OS01G0717400 | GCTCATCACGTCGTAGCCGAAGG | 4 | Not detected | |
OS01G0215000 | GCCTACCACTTCATTGCTGATGG | 4 | Not detected | |
OS09G0298500 | GCTGATCACTTCAAAGCCCCAAG | 4 | Not detected |