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Fig. 6 | Phytopathology Research

Fig. 6

From: The histone deacetylase Cfhos2 is a key epigenetic factor regulating appressorium development and pathogenesis in apple Glomerella leaf spot fungus Colletotrichum fructicola

Fig. 6

Infection of ΔCfhos2 mutant elicits stronger plant defense reactions. Infected apple leaves were sampled at 2–4 days post-inoculation (dpi). Fungal infectious hyphae were visualized with the aid of FITC-WGA staining. a At the early infection phase, WT differentiates elongated invasive hyphae (left) whereas ΔCfhos2 (Δhos2-4) differentiates spherical vesicles with distorted branches (right), the mutant also elicits cytoplasmic condensation or browning of apple epidermal cells (rightmost). b Infectious hyphae of ΔCfhos2 (Δhos2-4) induces stronger degree of mesophyll cell browning than WT (1104-6). c Distortion of infectious hyphae of the ΔCfhos2 (Δhos2-4) mutant. Scale bars in a and c represent 20 μm, scale bars in b represent 50 μm

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