Fig. 5

Penetration peg formation on cellophane. a Colonies of XS11 and mutants (ΔVdSep3, ΔVdSep4, ΔVdSep5, and ΔVdSep6) grown on minimal medium (MM) overlaid with (upper panel) or without a cellophane membrane (lower panel). The strains were grown on MM plates overlaid with a cellophane membrane at 25 °C for 3 days or 6 days, and incubated for another 3 days after the membrane was removed. Bar = 2 cm. b Hyphopodia and penetration pegs formed on a cellophane membrane at 6 dpi. Bar = 10 μm. c Column diagram showing the number of penetration pegs formed by each strain on cellophane. The error bars represent standard deviations based on three independent replicates (**, P < 0.01)