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Fig. 7 | Phytopathology Research

Fig. 7

From: Sterol demethylation inhibitor fungicide resistance in Colletotrichum siamense from chili is caused by mutations in CYP51A and CYP51B

Fig. 7

Construction and identification of CYP51A or CYP51B replacement mutants of Colletotrichum siamense. a Strategies for the construction of CYP51A or CYP51B replacement mutants. The CYP51A and CYP51B replacement vectors contain the hygromycin phosphotransferase gene (HPH) and geneticin resistance gene (NEO), respectively. The primers used are indicated by arrows, and asterisks indicate the mutations in CYP51A and CYP51B. b PCR identification of the CYP51A replacement mutants. Fragments of 2.97 kb and 1.87 kb could be amplified from the CYP51A replacement mutants with the primer pair F4/R4 and F5/R5, respectively, but could not be amplified from the sensitive isolates. c PCR identification of the CYP51B replacement mutants. Fragments of 2.09 kb and 1.95 kb could be amplified from the CYP51B replacement mutants with the primer pair F10/R10 and F11/R11, respectively, but could not be amplified from the sensitive isolates

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