Sterol demethylation inhibitor fungicide resistance in Colletotrichum siamense from chili is caused by mutations in CYP51A and CYP51B

Table 5 Protective activity of tebuconazole against Colletotrichum siamense isolates on chili fruits

Isolates	0 mg/L tebuconazole		50 mg/L tebuconazole		100 mg/L tebuconazole		200 mg/L tebuconazole
Isolates	Lesion size (mm)^a	Protective efficiency (%)^b	Lesion size (mm)	Protective efficiency (%)	Lesion size (mm)	Protective efficiency (%)	Lesion size (mm)	Protective efficiency (%)
Zz14	10.85 ± 0.95a	–	7.02 ± 0.23a	35.29 ± 2.52a	2.11 ± 0.56b	80.55 ± 1.12a	ND^c		100a
Zz9	8.56 ± 0.46b	–	6.54 ± 0.15b	23.59 ± 1.35c	2.56 ± 0.35b	70.09 ± 2.23b	1.56 ± 0.31b		81.77 ± 2.11b
Zz5	8.79 ± 0.33b	–	7.61 ± 0.45a	13.42 ± 1.65d	4.32 ± 0.12a	50.85 ± 2.63e	2.86 ± 0.45a		67.46 ± 3.21d
Zz11	6.77 ± 0.14c	–	4.78 ± 0.17c	29.39 ± 2.45b	2.14 ± 0.15b	68.39 ± 1.95c	1.76 ± 0.13b		74.00 ± 3.31c
Zz12	6.89 ± 0.24c	–	4.63 ± 0.25c	32.80 ± 2.65a	2.40 ± 0.31b	65.16 ± 2.89d	1.54 ± 0.16b		77.64 ± 1.12c
Zz3	5.98 ± 0.43d	–	3.87 ± 0.37d	34.29 ± 1.86a	2.05 ± 0.24b	65.19 ± 1.67d	1.32 ± 0.27b		77.58 ± 2.36bc

^aValues are the means ± standard deviations from 15 replicate chili fruits. Different letters indicate significant differences according to the Fisher’s LSD test at P = 0.05. ^bProtective efficacy (%) = [(lesion size of control − lesion size of treatment)/lesion size of control] × 100. ^cND, not detected