Fig. 1
From: Identification of a novel C6 protein encoded by tomato leaf curl China virus
Detection and sequence analyses of a begomovirus-encoded C6 protein. a Detection of unique C6 peptides by mass spectrometry analysis in Nicotiana benthamiana plants infected with ToLCCNV/ToLCCNB. b MS spectrum identification of the unique-peptides 74MLDKLQVLNCEHTPKTK90 and 1MGRLAHAFDFDMAGAIR17 in the ToLCCNV-C6 protein. c Schematic representation of the genome organization of ToLCCNV. d Western blot showing GFP protein accumulation at 2 days post-inoculation (dpi). Agrobacterium tumefaciens clones harboring pCambia-GFP or pC6 (556)-GFP at an OD600 = 1.0, or 35S-GFP at an OD600 = 0.05 were individually inoculated in N. benthamiana leaves. Actin serves as a loading control. e A. tumefaciens clone harboring pC6(556)-GFP was inoculated in N. benthamiana leaves in the presence of ToLCCNV/ToLCCNB or pBinplus. At 3 dpi, GFP fluorescence was measured and processed with the same settings. Scale bars: 25 μm. f Western blot showing GFP protein accumulation at 3 dpi. A. tumefaciens clone harboring pC6 (556)-GFP was co-infiltrated with ToLCCNV/ToLCCNB infectious clones or pBinplus vector in N. benthamiana leaves. Ponceau S staining of Rubisco shows protein loading. Numbers indicate the average GFP accumulation of three independent biological replicates