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Fig. 5 | Phytopathology Research

Fig. 5

From: Identification of a novel C6 protein encoded by tomato leaf curl China virus

Fig. 5

ToLCCNV C6 protein is dispensable for virulence. a Symptoms in Nicotiana benthamiana plants at 8 days post-inoculation (dpi) with PVX-C6 or PVX-mC6. b Western blot analysis of PVX coat protein (PVX-CP) accumulation in plants infected with PVX-C6 or PVX-mC6. Ponceau S staining of Rubisco shows protein loading. c Quantification of PVX-CP accumulation in plants infected with PVX-C6 or PVX-mC6, analyzed from the Western blot images using Image J. Data are the mean of seven independent biological replicates. d Symptoms of N. benthamiana plants infected with ToLCCNV/ToLCCNB, ToLCCNV-mC6/ToLCCNB, or control (pBinplus) at 8 dpi. e Western blot analysis of ToLCCNV coat protein (CP) accumulation in systemic leaves shown in d. Ponceau S staining of Rubisco shows protein loading. f Quantification of CP accumulation in N. benthamiana plants infected with ToLCCNV/ToLCCNB or ToLCCNV-mC6/ToLCCNB, analyzed from the Western blot images using Image J. Data are the mean of six independent biological replicates. g Viral DNA accumulation in ToLCCNV/ToLCCNB, ToLCCNV-mC6/ToLCCNB infected or control (pBinplus) plants in d, measured by qPCR. Data are the mean of six independent biological replicates. Error bars represent standard deviation (SD). ns, no significant difference (Student′s t-test; P ≥ 0.05). NbActin was used as an internal reference

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