Fig. 1

Analyzation of XLG proteins in Nicotiana benthamiana. a Phylogenetic analysis of Gα proteins from N. benthamiana and Arabidopsis. b Silencing of NbXLG3,5 or NbXLG4 leads to enhanced susceptibility to Sclerotinia sclerotiorum. The indicated NbXLG genes were silenced by virus-induced gene silencing (VIGS). The plants were inoculated with S. sclerotiorum and photographed under UV light at 24 h post-inoculation (hpi). The lesion areas were measured and calculated (mean ± SD, n ≥ 6, student’s t test, **significant difference at P < 0.01). Scale bar, 1 cm. c Silencing of NbXLG3,5 or NbXLG4 leads to compromised flg22-induced ROS production. The indicated NbXLG-silenced plants were subjected to flg22-induced ROS examination (mean ± SD, n ≥ 6, student’s t test, **significant difference at P < 0.01). RLU, relative luminescence units. d Flg22 and chitin induce protein band shift of NbXLG3, NbXLG4, and NbXLG5 in the N-terminus. The N-terminal ~ 200 amino acid of NbXLGs were transiently expressed in N. benthamiana for 2 days, infiltrated with 1 μM flg22, or 200 μg/mL chitin for 10 min, and protein band shift was examined by anti-HA immunoblots