Fig. 2

Generation of Nbxlg3,5- or Nbxlg4-knockout mutants. a Knocking out of NbXLG3,5 or NbXLG4 via CRISPR/Cas9 approach. In the schematic map, the exons, introns, and UTRs are indicated as yellow boxes, lines, and bule boxes, respectively. The gRNA sequences and gene editing results are listed in the diagram. The DNA-sequencing chromatograms are shown in Additional file 1: Figure S3. b Morphologic phenotypes of 20- and 40- days-old NbXLG3,5 and Nbxlg4 mutant plants. Bar, 1 cm