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Fig. 5 | Phytopathology Research

Fig. 5

From: Extra-large G proteins regulate disease resistance by directly coupling to immune receptors in Nicotiana benthamiana

Fig. 5

NbXLG3, NbXLG4, and NbXLG5 regulate flg22- or chitin-induced ROS and defence gene expression by coupling to plant immune receptors. a, b The Nbxlg3,5 and Nbxlg4 mutant lines showed significantly reduced ROS burst upon flg22 (a) or chitin (b) treatment. The indicated plants were examined for flg22- or chitin-induced ROS burst and the peak RLU value were recorded (mean ± SD, n ≥ 6, student’s t test, **significant difference at P < 0.01). c, d Flg22- or chitin- induced expression of defence marker gene ACRE31 and PTI5 are compromised in Nbxlg3,5 and Nbxlg4 mutant lines (Mean ± SD; student’s t test, **significant difference at P < 0.01.). e, f NbXLG3/5 and NbXLG4 interact with NbFLS2 (e) and NbCERK1(f). The indicated constructs were transiently expressed in Nicotiana benthamiana and protein interactions were examined by luciferase complementation image (LCI) assays (mean ± SD; n ≥ 6, student’s t test, **significant difference at P < 0.01). In a, b, e, and f, RLU indicates relative luminescence units

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