Table 6 Primers used in the study
Primer | Sequence | Description | Reference |
|---|---|---|---|
CQ176 | F:5′-CGGTCCGGTCAACCTACTAC-3’ | Amplify ~ 360 bp amplicons from the Stagonosporopsis citrulli citrulli isolate | Stewart et al. (2015) |
CQ177 | R:5′-CACGCCAGCAAATCACACTA-’ | ||
CQ178 | F:5′-GGTGACATCTTGCGTGAATG-3′ | Amplify ~ 270 bp amplicons from the S. citrulli and S. cucurbitacearum isolates | |
CQ179 | R:5′-TGGTTGTTTGGTTGTTTGGA-3′ | ||
CQ180 | F:5′-TATGACGTTGGGCAAGTGAG-′ | Amplify ~ 220 bp amplicons from the S. caricae, S. citrulli and S. cucurbitacearum isolates | |
CQ181 | R:5′-TTTGCTGGGATGGTGTTGTA-3′ | ||
CQ82 | F:5′-TCCGCCGTTCCTCATTG-3′ | Sequencing of the cyp51 coding region | This study |
CQ83 | R:5′-GCTGCCGTTCACATTCC-3′ | ||
CQ146 | F:5′-CAGAACCCTGGTCTTATTG-3′ | Determination of the cyp51 gene expression | This study |
CQ147 | R:5′-GAGGCTGCTTGACTTTAC-3′ |