Fig. 2
Topical application of gene-targeting dsRNA synthesized in vitro inhibited the virulence of Rhizoctonia solani. a Synthesis of RsAGO1/2-dsRNA in vitro. Lane M: DL5000 DNA Marker; Lane Template: the DNA template of RsAGO1/2-dsRNA; Lane PC: replacing RsAGO1/2 into control template in kit; Lane NC: system without T7 RNA Polymerase. b Rice leaves were inoculated with mycelium plugs of R. solani (5 mm diameter) and treated with RsAGO1/2-dsRNA on the same site (dsRNA: 50 ng/μL). Water and YFP-dsRNA were used as controls. c The impact of RsAGO1/2-dsRNA on the expression of RsAGO1 and RsAGO2 genes. Relative transcript levels of RsAGO1 and RsAGO2 were determined using RT-qPCR. Data are means ± SD from three replicates. Data are means ± SD of three replicates. **Indicates significant difference at P < 0.01 (Student’s t-test)