Fig. 4

Enhanced stability of dsRNA loading into nanoparticles. a Fluorescence signals of 5 μL reaction solutions (dsRNA: 50 ng/μL) and controls (water and YFP-dsRNA) before and after treatment with MNase for 30 min were analyzed using Zeiss inverted fluorescence microscope. Images of samples before and after treatment with MNase were taken, respectively. b Fluorescence intensities of nanoparticle-dsRNA complexes before and after treatment with MNase were assayed using ImageJ software. Data are means ± SD of three replicates. **Indicates significant difference at P < 0.01 (Student’s t-test)