Skip to main content
Fig. 5 | Phytopathology Research

Fig. 5

From: The recombination regulator RecX negatively regulates heat-stable antifungal factor (HSAF) biosynthesis in Lysobacter enzymogenes

Fig. 5

RecX directly bound the promoter of the HSAF biosynthesis gene (plafB). a Direct physical interaction between RecX and the lafB promoter region was detected in E. coli. Experiments were performed according to the procedures described in the ‘Experimental procedures’ section. + , cotransformant containing pBX-R2031 and pTRG-R3133, used as a positive control; − , cotransformant containing pBXcmT-plafB and the empty pTRG, used as a negative control; L. enzymogenes RecX and plafB, cotransformant harboring pTRG-RecX and pBXcmT-plafB. -3AT-Str, plate with no selective medium (3AT 3-amino-1,2,4-triazole and Str streptomycin) and + 3AT + Str, plate with M9-based selective medium. b The purified protein was analyzed by 12% SDS-PAGE. Lane 1, molecular mass markers; Lane 2, RecX protein. c Gel shift assay showing that RecX directly bound plafB. RecX (0, 0.5, 1, 2, or 4 μM) was added to reaction mixtures containing 50 ng of probe DNA, and the reaction mixtures were separated on polyacrylamide gels

Back to article page