Fig. 4
From: 3′ UTR is critical for viral RNA accumulation of jasmine virus H
Pathogenicity assessment of recombinant viruses constructed based on field symptoms difference. a Typical mosaic was observed on jasmine samples from Fujian but not Jilin Province. b Relative quantification of viral RNA by real time qPCR in jasmine leaves showed in a, relative abundance of JaVH was estimated by the 2−∆CT method using actin as control. c Schematic diagram of recombinant plasmids. The yellow box represents sequences related to JaVH-FJ while the blue box represents sequences related to JaVH-JL. d Detection of genomic RNA, subgenomic RNA, and virus derived siRNA of JaVH by Northern blotting and CP by Western blotting at 15 days post-infiltration (dpi). A size marker and loading control are shown for reference