Fig. 3

Effect of Tm-2² allele composition of tomato cultivars on the disease resistance against ToMMV. a The symptoms of systemic leaves of tomato cultivars Jinpeng 1 (Tm-2²/tm-2) and Chaobei (Tm-2²/Tm-2) inoculated with ToMMV or ToBRFV at 14 days post-agro-infiltration. b, c RT-PCR analysis of ToMMV and ToBRFV RNA in the systemic leaves of tomato cultivars Jinpeng 1 and Chaobei. UBI gene was used as an internal control. This experiment was repeated three times. d Alignment of partial coding sequences of tm-2 (accession number; AF536199.1), Tm-2 (accession number; AF536200.1), and Tm-2² (accession number; AF536201.1). e Sequence chromatogram showing the allele composition of tomato cultivars Jinpeng 1 and Chaobei. f Hypersensitive response-associated cell death in wild-type Nicotiana benthamiana leaves co-expressed with ToMMV MP and Tm-2², Tm-2, or tm-2. Tm-2²-Chaobei and Tm-2-Chaobei were derived from Chaobei tomato and Tm-2²-Jinpeng 1 or tm-2-Jinpeng 1 was derived from Jinpeng 1 tomato. The picture was taken at 48 h post-agro-infiltration. Yellow arrows show symptomatic systemic leaves and white arrows show asymptomatic systemic leaves