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Fig. 4 | Phytopathology Research

Fig. 4

From: The role of different innate and environmental factors in Tm-22-mediated resistance to tomato mottle mosaic virus

Fig. 4

Effects of Tm-22 accumulation on the disease resistance against ToMMV. a RT-qPCR analysis of the Tm-22 mRNA accumulation levels in the silenced and control Tm-22 transgenic Nicotiana benthamiana plants inoculated with TRV-derived vector at 10 days post-agro-infiltration (dpai). Yellow arrows show symptomatic systemic leaves and white arrows show asymptomatic systemic leaves. b The symptoms of Tm-22-silenced and the control Tm-22 transgenic N. benthamiana plants inoculated with ToMMV at 7 dpai. c RT-PCR detection of ToMMV RNA in the systemic leaves of the silenced and the control Tm-22 transgenic N. benthamiana plants. d RT-qPCR analysis of Tm-22 mRNA accumulation levels in the silenced and the control tomato cultivar Jili inoculated TRV-derived vector at 10 dpai. e The symptoms of Tm-22-silenced and control Jili tomato plants inoculated with ToMMV at 14 dpai. f RT-PCR detection of ToMMV RNA accumulation in systemic leaves of Tm-2.2-silenced and control plants of tomato cultivar Jili. The mRNA accumulation of the UBI gene served as an internal control. The values indicate the mean + SD of three biological replicates. Letters indicate significant differences in different treatments (P < 0.05)

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