Fig. 6
Effect of temperature on the Tm-22-mediated disease resistance against ToMMV. a The symptoms of tomato cultivars Jili (upper panel) and susceptible Moneymaker (lower panel) inoculated with ToMMV and exposed to temperatures of 20, 25, 30, and 35°C at 14 days post-inoculation (dpi). b, c RT-PCR detection of ToMMV RNA in systemic leaves of tomato cultivars Jili and Moneymaker respectively exposed to different temperatures. d The symptoms of Tm-22 transgenic (upper panel) and wild-type (lower panel) Nicotiana benthamiana plants inoculated with ToMMV and exposed to temperatures of 20, 25, 30, and 35°C at 7 dpi. e, f RT-PCR detection of ToMMV RNA in the systemic leaves of the Tm-22 transgenic and wild-type N. benthamiana plants. The numbers in the lower right corner of each picture indicate the number of infected plants out of the total inoculated plants for three experimental repeats. The yellow arrows show symptomatic systemic leaves and white arrows show asymptomatic systemic leaves