Molecular evidences for population differentiation and the migration from south to north of Puccinia triticina in eastern China

Li, Hongfu; Zhang, Qinqin; Wang, Gui; Wang, Jifeng; Chen, Zhiyong; Zhao, Wuchao; Zheng, Xinyue; Gao, Li; Liu, Bo; Xu, Lijian; Chen, Wanquan; Liu, Taiguo

doi:10.1186/s42483-023-00163-3

Table 1 Sequence information, number of alleles (N_A), observed heterozygosity (H_O), expected heterozygosity (He), and unbiased expected heterozygosity (uHe) of 16 codominant simple sequence repeat (SSR) markers employed in this study

From: Molecular evidences for population differentiation and the migration from south to north of Puccinia triticina in eastern China

Locus	EMBL	Repeat motif	Primer sequence (5'–3')	Ta (℃)	N_A (size: bp)	H_O	H_E	uHe	Reference
RB17	AJ508886	(TGC)₅ + (TGG)₆	F: CTTCGGTAGGATTTCGAGCG	58	6 (192–200)	0.067 ± 0.040	0.127 ± 0.061	0.129 ± 0.062	Duan et al. (2003)
			R: CAGCTCCAAATCCTTTGCC
RB28	AJ508890	(TGG)₅	F: CATCTGGCTGGTGAGGTCGC	58	8 (319–335)	0 ± 0.008	0.278 ± 0.045	0.282 ± 0.046	Duan et al. (2003)
			R: GAAGCCCGCCGAGCAGC
RB4	AJ508880	(GT)₈	F: CAGTATTGTGGTGGTTGGATG	60	6 (247–256)	1.000 ± 0.016	0.5 ± 0.017	0.508 ± 0.017	Duan et al. (2003)
			R: ACTCAAGAATAATGGGGAACAC
RB12	AJ508884	(AG)₅₊₃	F: CCACAAGCAACCACATACCACC	58	11 (266–300)	0.800 ± 0.049	0.491 ± 0.027	0.499 ± 0.028	Duan et al. (2003)
			R: TGGTCCATGAAGAAGTCTCTGAAC
RB8	AJ508881	(TGG)₇	F: CGCCGTTCCCATCGTTC	60	5 (143–155)	0.267 ± 0.087	0.32 ± 0.062	0.325 ± 0.062	Duan et al. (2003)
			R: TAAAACACTCCACCCACGCC
RB1	AJ508879	(GT)₅	F: TTGTCGTTCTGGAATGATGC	58	6 (127–139)	0.833 ± 0.046	0.486 ± 0.008	0.494 ± 0.008	Duan et al. (2003)
			R: TGCCCACAACCCTCCTC
RB35	AJ508893	(AC)₉ + (TA)₅ + (AG)₅	F: ACTGCGATATCCAGTACACACAC	60	6 (245–250)	0.700 ± 0.062	0.654 ± 0.027	0.665 ± 0.027	Duan et al. (2003)
			R: TGATGGGCTCGCAGTGG
PtSSR151A	DQ789156	(AAC)₁₂	F: TCATCGCACTCCACTCAGAC	61	4 (469–475)	0.667 ± 0.080	0.464 ± 0.031	0.472 ± 0.032	Szabo and Kolmer (2007)
			R: ATGCTGCCCAACCTGCTC
PtSSR161	DQ789160	(TC)₁₃	F: ACTGCCTCCTGTGCCTTCT	60	6 (217–222)	0.900 ± 0.044	0.509 ± 0.021	0.518 ± 0.021	Szabo and Kolmer (2007)
			R: TAGTCCGAGGGTGACGAAGT
RB11	AJ508883	(CA)₁₇	F: AGCAGTGAGCAGCAGCGTC	60	6 (176–202)	0.833 ± 0.035	0.486 ± 0.024	0.494 ± 0.024	Duan et al. (2003)
			R: ACTACTGTGAGTGTCGGCTTGG
PtSSR152	DQ789157	(TG)₇ + (TG)₃ + (TG)₃	F: CTCCGTTCCTCTTTCTGTCG	61	4 (380–393)	0.233 ± 0.071	0.255 ± 0.044	0.259 ± 0.044	Szabo and Kolmer (2007)
			R: CCATCGCAACCAACAAACA
PtSSR173	DQ789162	(TC)₃ + (TC)₁₂	F: CTCAGCGACCTCAAAGAACC	60	6 (212–222)	0.533 ± 0.077	0.475 ± 0.045	0.483 ± 0.046	Szabo and Kolmer (2007)
			R: GAGACGACGGATGTCAACAA
PtSSR164	DQ789161	(TC)₁₃	F: GTGGAAGTGAGCGGAAGAAG	61	5 (218–228)	0.567 ± 0.068	0.459 ± 0.025	0.467 ± 0.026	Szabo and Kolmer (2007)
			R: GGAGATGGGCAGATGAGGTA
PtSSR55	DQ789150	(TC)₁₀	F: AGCTTACGGTCCTCAATCG	61	3 (304–308)	0.567 ± 0.064	0.406 ± 0.024	0.413 ± 0.024	Szabo and Kolmer (2007)
			R: AGTGAAAGGGGCTGGGAGT
PtSSR13	DQ789148	(TC)₉	F: CGAATTCGCGTTTTATGTCC	61	5 (125–130)	0.567 ± 0.092	0.663 ± 0.042	0.674 ± 0.042	Szabo and Kolmer (2007)
			R: TGATCCAATCGAACCTAGCC
RB16	AJ508885	(TGG)₇ + (GT)₄	F: CATTTGAGCCACGGTTGACTG	61	3 (281–284)	0 ± 0.051	0.278 ± 0.052	0.282 ± 0.053	Duan et al. (2003)
			R: AGACATGGTCGCAGCCACTG
Mean					5.69	0.588 ± 0.026	0.440 ± 0.013	0.448 ± 0.014

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