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Fig. 2 | Phytopathology Research

Fig. 2

From: Candidatus Phytoplasma ziziphi encodes non-classically secreted proteins that suppress hypersensitive cell death response in Nicotiana benthamiana

Fig. 2

Validation of the putative ncSecPs of Candidatus Phytoplasma ziziphi by using the Escherichia coli-based PhoA assays. a Diagrams of prokaryotic expression cassettes for phoA and its related fusion genes. The pET-phoA harboring the full-length phoA gene was used as the positive control, while pET-mphoA containing mphoA without a signal peptide (SP)-encoding sequence was used as the negative control. The Ca. P. ziziphin ncSecP was cloned into pET-mphoA and fused with mphoA, resulting in pET-ncSecP-mphoA. T7 indicates T7 promoter, and Ter represents T7 terminator. b PhoA assays verified the secretion of the in silico predicted Ca. P. ziziphin ncSecPs. The E. coli cells expressing phoA fusions were grown on the indicator LB medium, as described in the ‘Methods’ section. The images were taken after 12 and 24 h of incubation. Twenty-five of 37 candidates were clarified to be ncSecPs. Representatives of the ncSecPs with strong (ncSecP7), medium (ncSecP2), and weak (ncSecP1) secretion activities are shown

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Phytopathology Research

ISSN: 2524-4167