Fig. 7
From: Leafhopper transmits soybean stay-green associated virus to leguminous plants
Common brown leafhopper (Orosius orientalis) transmits SoSGV to other leguminous plants. a Image of an O. orientalis nymph feeding on a soybean leaf. b PCR detecting SoSGV in different varieties of wild soybean Glycine soja infested or non-infested by nymphs of the common brown leafhoppers. SoSGV was amplified using primer pair #3133 and #3144. The gene coding for β-tubulin (GsTUBB) was amplified via PCR as a loading control. Water and a plasmid containing SoSGV genome DNA were used as negative (N) and positive (P) controls, respectively. Different lanes represent different plants tested using PCR. c Leaf symptoms of wild soybean variety 'Yong-40' at 14 days post-O. orientalis infestation. d PCR detection of SoSGV in a Vigna angularis landrace. See further details in panel b. e Leaf symptoms of V. angularis at 15 days post-O. orientalis infestation. f PCR detection of SoSGV in cowpea (V. unguiculata) cv. 'Te Xuan Zhang Tang Wang'. See further details in panel b