Fig. 4

The defense protein SlPR1 exerts no antifungal activation. a Expression profile of SlPR1 in tomato roots after Fol inoculation at the indicated times, as measured by FRPKM data. The data represent the means ± SEs of three independent replicates. (Student’s t-test; ns, no significant difference; ***P < 0.001; ****P < 0.0001). b Schematic representation of an SlPR1 model based on the predicted protein structure (left and right rotated 180° around the longitudinal axis). The multiple conserved motifs are marked as detailed in Additional file 2: Figure S4. c CBB-stained SDS-PAGE gel showing the purity of obtained MBP and MBP-SlPR1. d Antimicrobial activity of MBP-SlPR1 recombinant protein. After the addition of 200 μg/mL purified MBP-SlPR1 or MBP to 1 × 10⁶ Fol spores/mL to 5% YEPD media, the microtiter plates were incubated on a shaking platform (100 rpm, 25°C). The OD₆₀₀ was used to evaluate the antimicrobial activity. The data represent the means ± SEs (n = 4). e Inhibition of Fol growth in the presence of 200 μg/mL purified MBP-SlPR1 or MBP proteins. f The diameter of Fol colonies obtained from the samples in e. Statistical significance was revealed by Student’s t-test. (Ns, no significant difference)