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Fig. 6 | Phytopathology Research

Fig. 6

From: Enhancing tomato resistance by exploring early defense events against Fusarium wilt disease

Fig. 6

The CAPE1 peptide actives tomato leaf defense and systemic resistance. a Production of ROS in tomato leaves treated with 250 nM CAPE1 or 50 nM flg22. The mean values (±SEM) of eight replicates are shown. The experiments were repeated three times with similar results. b CAPE1 induced leaf H2O2 production. Detached tomato leaves were inoculated with water (mock) or 250 nM CAPE1 for 4 h. DAB staining was used to visualize H2O2 accumulation. The images are representative of the results of three biological repeats (left panel), and the amount of H2O2 was also quantified (right panel). c The levels of SA and JA in tomato leaves treated with CAPE1. The tomato leaves were sprayed with 250 nM CAPE1 or water (mock), and after 4 h, the content of SA and JA in leaves was quantified. d Hierarchical clustering of the abundance of transcripts of SA or JA signaling pathway genes in tomato leaves and roots. After CAPE1 pretreatment for 2 and 4 h, the leaves and roots were collected and subjected to qRT-PCR assays. e Fol resistance of tomato plants whose leaves were sprayed with a CAPE1 solution. Root inoculation was carried out 4 h after application of 250 nM synthetic CAPE1 or water (mock) to the tomato leaves. The disease symptoms were observed at 10 dpi. f The fresh weight of 10 tomato plants shown in e was measured at 10 dpi. Statistical significance was determined by Student’s t-test. The P values are shown

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