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Fig. 2 | Phytopathology Research

Fig. 2

From: Engineer complete resistance to Cotton Leaf Curl Multan virus by the CRISPR/Cas9 system in Nicotiana benthamiana

Fig. 2

Schematic representation of the experimental design of fsGFP assay and vector for generation of transgenic plants. a fsGFP assay to determine gRNA activity. b Schematic diagram of T-DNA region in a vector for generation of transgenic N. benthamiana plants. Arabidopsis U6 promoter is used to direct the expression of gRNA. oCas9, Arabidopsis codon-optimized Cas9; LB, left border; RB, right border; Ter, terminator; HptII, hygromycin phosphotransferase II

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Phytopathology Research

ISSN: 2524-4167