Fig. 1
From: Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses
Interaction of Pns11 with gelsolin in Y2H and Sf9 systems. a Schematic representation of the gelsolin gene with the domain of the gelsolin superfamily and other binding sites. b Phylogenetic relationships of gelsolin orthologs of N. cincticeps with counterparts. The available sequences were aligned using Clustal W, and phylogenetic trees were reconstructed using the neighbor-joining analysis with P-distance using MEGA 5.0. Reliability of the phylogenetic trees was estimated by calculating bootstrap confidence limits based on 1000 replicates. c Y2H assay of Pns11-gelsolin interaction. Transformants on plate of SD/−Leu-Trp-His-Ade medium were as follows: +, positive control, i.e., pTSU2-APP/pNubG-Fe65; −, negative control, i.e., pTSU2-APP/pPR3-N. d Co-localization of Pns11 and gelsolin in baculovirus expression system. Sf9 cells infected with recombinant baculoviruses encoding Pns11 or gelsolin were fixed at 48 hpi and prepared for immunofluorescence microscopy. Cells were immunostained with Pns11-FITC for Pns11, or the monoclonal antibody against strep tag and the secondary antibody of anti-mouse IgG-rhodamine for gelsolin. White arrows indicate the co-localization of Pns11 and gelsolin. Bars, 10 μm