Fig. 4

The PCR assay of the full-length cDNA libraries of upregulated genes. a The PCR amplified inserts from the pTriplEx2 library. Using the library plasmids as template, the insert fragments were amplified by vector-specific primers. b Size fractionation of cDNA inserts. Lanes 1–3, different ranges (0.5–1 kb, 1–1.5 kb, > 1.5 kb) of fractionated cDNA inserts. c Gene-specific analysis. Using plasmids of the expression library I as templates, reference genes were amplified by gene-specific primers. The products were analyzed on 1% agarose gels as indicated. d and e Sizes of the library inserts. Randomly selected 48 colonies from library I (d) and II (e) were used as templates, and vector-specific primers were used for PCR. The arrows indicate the bands of expected size when two distinct bands appeared. Lane M₁, DNA ladder (Generay. Code No. 1709G22). Lane M, DNA ladder (Generay. Code No. 1705G22)