Fig. 7

Spatial and temporal changes of Tri4 upon induction in vitro and in planta. a The growing hyphae of Tri4-GFP strain exhibit a reticulate fluorescence pattern on solid starch yeast media (SYM), while it switches to crescent and spherical perinuclear pattern in liquid trichothecene biosynthesis (TBI) media. b The Tri4-GFP is poorly expressed in invasive hyphae at early proliferation stage (12 hpi). In swelling invasive hyphae, the Tri4-GFP develops clear crescent and spherical perinuclear structures at 24 hpi. At 36 hpi, extensively grown fungal invasive hyphae were seen in the wheat coleoptiles cells with some of these hyphae expressing Tri4-GFP. c Relative expression levels of Tri4 at the indicated time points after inoculation were determined by reverse transcriptase-quantitative polymerase chain reaction. Means ± SE were calculated from the results of three replications