Aphid vectors impose a major bottleneck on Soybean dwarf virus populations for horizontal transmission in soybean

Table 2 Genetic variation in SbDV-MD6 populations in different host species and passages

Host and passage	Mutated clones	Total mutations/ bases sequenced	Mutation frequency (%)
Clover	60% (6/10)	25/29, 590	0.08^a
Pea and passage 1	55% (11/20)	66/59, 180	0.11^a
Pea and passage 8	40% (8/20)	64/59, 180	0.10^a
Soybean and passage 1	56% (10/18)	64/53, 262	0.12^b
Soybean and passage 6	44% (8/18)	53/53, 262	0.10^a

Substitutions are counted for determining mutation frequency. Mutation frequencies with the same letter were not statistically different. Least significant differences were determined by the ANOVA test (P < 0.05)