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Fig. 7 | Phytopathology Research

Fig. 7

From: A putative N-BAR-domain protein is crucially required for the development of hyphae tip appressorium-like structure and its plant infection in Magnaporthe oryzae

Fig. 7

BAR domain-containing proteins regulate the expression of membrane tubulation related genes. a The inhibitory effects of cell oxidative and reductive stress-inducing osmolytes on the vegetative growth of the ΔMobar-A, ΔMobar-B, ΔMobar-AMobar-B, and the wild-type strains cultured on CM media supplemented independently with 2 mM DTT, 0.7 M NaCl, 200 μg/mL Calcofluor White (CFW), 0.01% SDS and 200 μg/mL Conge Red (CR) for 10 days. b Growth responses of ΔMobar-A, ΔMobar-B, ΔMobar-AMobar-B, and the wild-type strains to the different oxidative and reductive stress-inducing osmolytes. Error bars represent standard errors from three independent replicates (*, P < 0.05 by t-test). c The expression levels of putative membrane and membrane-associated genes in the ΔMobar-A, ΔMobar-B, and ΔMobar-AMobar-B strains during vegetative growth. The qPCR results were obtained from three independent biological replications with three technical replicates. Error bars represent standard errors (*, P < 0.05; **, P < 0.01 by t-test). d Yeast-two-hybrid assay represent protein-protein level interaction between MoBar-A and putative membrane associated proteins in M. oryzae. e The interaction pattern of MoBar-B with putative membrane associated proteins in M. oryzae

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