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Fig. 1 | Phytopathology Research

Fig. 1

From: A novel gene from a secondary metabolism gene cluster is required for microsclerotia formation and virulence in Verticillium dahliae

Fig. 1

Identification of a novel unannotated gene with RNA-Seq data and reverse-transcription PCR. a Mapping of RNA-Seq reads revealed a non-annotated transcribed region in the genome of V. dahliae (dotted red box). CO and GC represent conidia and germinated conidia (at 12 h), MS1-MS4 represent four typical stages during the entire process of microsclerotial formation at 60 h, 72 h, 96 h and 14 days. b Schematic diagram of the structure of the novel gene. Arrow shows the direction of transcription. ATG indicates the start codon; TGA indicates the stop codon. P5 and P6 are primers. c Transcript evidence of the ORF fragment of the novel gene in different V. dahliae isolates. P5/P6 were the primers used for reverse-transcription PCR confirmation. d Schematic diagram of the domain architecture of the novel gene

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Phytopathology Research

ISSN: 2524-4167