Fig. 4

High Si content enhances H₂O₂ production in leaves of Col-0 plants expressing a heterologous silicon transporter. a, b, c and d Representative microscopic images showing growth of Gc UCSC1 at 5 dpi on leaves of the plants of the indicated genotypes grown in perlite supplemented with 0 mM (−Si) or 1.0 mM (+Si) Si. Production and in situ accumulation of H₂O₂ was visualized as reddish-brown stain upon reaction with 3,3-diaminobenzidine, while fungal structures were stained blue by trypan blue. Scale bars = 200 μm