Fig. 4From: Development of polyclonal antisera against movement proteins from three poleroviruses infecting cucurbitsSpecificity analysis of antisera by western blotting. The left lane is the pageruler prestained protein ladder marker, and the other lanes are proteins extracted from N. benthamiana leaves, transiently expressing different MPs including CABYV, MABYV, SABYV, BrYV, PLRV and ScYLV. pMDC32 empty vector is a negative control. The three test antisera, anti-MPCABYV (a), anti-MPMABYV (b) and anti-MPSABYV (c) were used at 1:1000, 1:10000 and 1:20000Back to article page