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Table 1 Advantages and disadvantages of plant positive-strand and negative-strand RNA virus-based vectors

From: Reverse genetics systems of plant negative-strand RNA viruses are difficult to be developed but powerful for virus-host interaction studies and virus-based vector applications

Types Advantages Disadvantages
Positive-strand RNA viral vector ✧ Without obvious immunogenicity in human;
✧ Consuming much less time and labor than transgenic methods;
✧ Applications in various plant species;
✧ Applications for transient expression.
✧ Easy manipulation on their simple genomes;
✧ Many available viral vectors.
♦ Used mostly on dicot plants;
♦ Incapable of accommodating large inserts.
Negative-strand RNA viral vector ✧ Simultaneous expression of multiple heterogonous proteins with high yields;
✧ Accommodation for large inserts;
✧ Allowing for genomic studies of insect vectors;
✧ Capable of co-delivery of gene editing reagents such as Cas9 and sgRNA.
♦ Very few infectious clones of plant NSR viruses can be found.