Table 2 Isolates utilized in sensitivity and specificity analyses and their origins
Species | Isolate number | Host | Source/Origin | Result of specificity analysis |
|---|---|---|---|---|
Phytophthora fragariae | MRV1 | Fragaria ananassa | Isolated from North Savo region of Finland by the University of Eastern Finland | Amplification onset at ~ 5 min |
Phytophthora fragariae | VTJ1 | Fragaria ananassa | Isolated from North Savo region of Finland by the University of Eastern Finland | Amplification onset at ~ 5 min |
Phytophthora fragariae | KRT1 | Fragaria ananassa | Isolated from North Savo region of Finland by the University of Eastern Finland | Amplification onset at ~ 5 min |
Phytophthora fragariae | SCRP245 fvf7 | Fragaria ananassa | Most probably isolated from United Kingdom | Amplification onset at ~ 5 min |
Phytophthora cactorum | PO245 | Fragaria ananassa | Isolated from Poland and donated by Grażyna Szkuta from Państwowa Inspekcja Ochrony Roślin i Nasiennictwa | No amplification |
Phytophthora taxon raspberry | GE5 | Unknown (species also confirmed through atp9-nad9 sequencing) | Unknown origin | No amplification |
Phytophthora megasperma | GE9 | Unknown (species also confirmed through atp9-nad9 sequencing) | Unknown origin | No amplification |
Phytophthora rosacearum | SO18 | Unknown (species also confirmed through atp9-nad9 sequencing) | Unknown origin | No amplification |
Phytophthora ramorum | Ph426 | Rhododendron catawbiense ‘Grandiflorum’ | Isolated from Finland and donated by Anna Poimala from the Natural Resources Institute Finland (Luke) | No amplification |
Phytophthora plurivora | Ph441 | Rhododendron ‘Marketta’ | Isolated from Finland and donated by Anna Poimala from the Natural Resources Institute Finland (Luke) | No amplification |
Phytophthora pini | Ph443 | Rhododendron ‘Capistrano’ | Isolated from Finland and donated by Anna Poimala from the Natural Resources Institute Finland (Luke) | No amplification |
Phytophthora cambivora | BBA 21/95-K II | Chamaecyparis lawsoniana ‘Columnaris’ | Isolated from Germany and donated by Sabine Werres from Julius Kühn-Institut (JKI) | No amplification |
Phytophthora cinnamomi | BBA62660 | Rhododendron sp. | Isolated from Germany and donated by Sabine Werres from Julius Kühn-Institut (JKI) | No amplification |
Pythium sylvaticum | ISO-VTJC | Fragaria ananassa | Isolated from North Savo region of Finland by the University of Eastern Finland | No amplification |
Botrytis cinerea | ISO-57C | Fragaria ananassa | Isolated from North Savo region of Finland by the University of Eastern Finland | No amplification |
Colletotrichum acutatum | PCF753 | Fragaria ananassa | Isolated from Belgium and DNA donated by Jane Debode from the Institute for Agricultural, Fisheries and Food Research (ILVO) | No amplification |
Mucor hiemalis | ISO-125-MKR | Fragaria ananassa | Isolated from North Savo region of Finland by the University of Eastern Finland | No amplification |
Fusarium avenaceum | ISO2-125-MKR | Fragaria ananassa | Isolated from North Savo region of Finland by the University of Eastern Finland | No amplification |
Fusarium proliferatum | Tiste Koe 25 | Fragaria ananassa | Isolated from North Savo region of Finland by the University of Eastern Finland | No amplification |