Fig. 1
From: Rust effector PNPi interacting with wheat TaPR1a attenuates plant defense response
Validation of the protein interaction between PNPi and TaPR1a by Y2H and Co-IP assays. a Yeast transformants co-expressing different bait and prey constructs were assayed on SD-Leu-Trp-His-Ade selective medium. EV, empty vector. b A multiple sequence alignment was generated by MEGA software using CAPE1 regions of PR1 proteins from Solanum lycopersicum (Sl), Triticum aestivum (Ta), Hordeum vulgare (Hv), Nicotiana tabacum (Nt), Zea mays (Zm), and Oryza sativa (Os). c Schematic diagram of wheat transgenic construct. d Validation of the interaction between PNPi and TaPR1a by Co-IP assay. Total proteins from tobacco leaves transiently-expressing GFP-TaPR1a(25–164) and transgenic wheat line overexpressing SP(TaPR1a)-PNPi-cMYC were extracted and immunoprecipitated with an α-GFP antibody (α-GFP IP). Input and bound proteins were detected via immunoblot using α-GFP and α-cMYC antibodies, respectively