Table 2 GA reaction buffer (3.33×, 350 μL)
Reagent | Stock concentration | Volume | Final concentration |
|---|---|---|---|
Tris-HCl, pH 7.5a | 1000 mM | 115.5 μL | 330 mM |
MgCl2a | 2000 mM | 5.8 μL | 33 mM |
DTT | 1000 mM | 11.6 μL | 33 mM |
dNTP mix | 25 mM (each) | 9.2 μL | 0.66 mM |
NAD+ | 50 mM | 23.1 μL | 3.30 mM |
PEG-8000b | 35% | 165.0 μL | 16.50% |
n.f. dH2O | – | 19.8 μL | – |
Total volume | – | 350.0 μL | – |