Fig. 5

Validation of the RNA-Seq data by qRT-PCR. The relative expression levels of the indicated genes were verified by qRT-PCR with EF1α gene as a reference. T0 and T6 are the control plants (pTRV:GFP) without (T0) or with pathogen at 6 hpi (T6). E0 and E6 are the NbERF173-silenced plants without (E0) or with pathogen at 6 hpi (E6). Error bars represent the SD for three independent experiments (**P < 0.01 compared with GFP; Dunnett’s test). The left Y-axis represents the relative expression levels measured by qRT-PCR and the right means data of RNA-Seq