Table 1 Primers used for detection and amplification of tomato brown rugose fruit virus
Primer name | Primer sequences (5′ → 3′) |
|---|---|
ToBRFV-591-F | GACAGGTGAATGGAATTTGCCAGATAATTG |
ToBRFV-591-R | AGACATATTTAATACGAATCTGAATCGGCG |
ToBRFV-842-F | GAAGTCCCGATGTCTGTAAGG |
ToBRFV-842-R | GTGCCTACGGATGTGTATGA |
ToBRFV-F-23 | CAACAACAACAAACAACAAACAACA |
ToBRFV-R-1623 | CTAATGCGTCTCCCGACAC |
ToBRFV-F-1534 | AGATTTCCCTGGCTTTTGGA |
ToBRFV-R-3733 | ATCATCGCCACCAAATTTTC |
ToBRFV-F-3669 | ATGGTACGAACGGCGGCAG |
ToBRFV-R-4720 | CAATCCTTGATGTGTTTAGCAC |
ToBRFV-F-4570 | ACCTTATGTGGAATTTCGAGG |
ToBRFV-R-5717 | AGACATATTTAATACGAATCTGAATCGGCG |
5′ RACE-GSP1 | GATTACGCCAAGCTTCACCCTGCCTGTGTTTTCCACCTGCTG |
3’RACE-GSP2 | GATTACGCCAAGCTTATGACGCAACGGTGGCTATAAGGAGCGC |
pCB-ToBRFV-1F | AGTTCATTTCATTTGGAGAGGCCTGTATTTTTTACAACATATACCAA |
pCB-ToBRFV-1R | CCTCGAGAAAGCAGCGCCTCTTTATCAGACTGA |
pCB-ToBRFV-2F | CTCAGTCTGATAAAGAGGCGCTGCTTTCTCGAGGATAT |
pCB-ToBRFV-2R | GAGATGCCATGCCGACCCGGGTGGGCCCCTACCGGGGGTTC |