Biochemical and molecular characterization of Alternaria alternata isolates highly resistant to procymidone from broccoli and cabbage

Table 6 Primers used in this study

Primer set	Forward primer (5′-3′)	Reverse primer (5′-3′)	Description^a
1	TGTGGCTTGCGGTTATTC	CGACGGCAGTGACGATG	Amplification of AaHK1 from −128 to + 820
2	AGCCAGGAAGTGAATAAGCA	TTCGTCGAGTCTCATAAC	Amplification of AaHK1 from + 726 to + 2097
3	CAACAATGTCAACACTATGG’	CTTCTCCGAGCTCAGTAAAC	Amplification of AaHK1 from + 1914 to + 2705
4	GAATGACCCAACTTACGCT	CATAAGTACGCAATCGTAAC	Amplification of AaHK1 from + 2598 to + 3672
5	ATGACGACGCCCTGTCTACC	ATCCAATACACACAAAAATG	Amplification of AaHK1 from + 3551 to + 4505
6	TCCGTAGGTGAACCTGCGG	TCCTCCGCTTATTGATATGC	Amplification of ITS to identify A. alternata
7	GAGTCACGTTAYCGCASC	TGGCTKACGGARACG	Amplification of KOG1058 to identify A. alternata
8	ACGCTTCTCATCTCCAAGATCCGT	AGAGAGCCTCGTTGTCAATGCAGA	Amplification of β-tubulin for qPCR
9	CTTACAAGGTGCCCGACTAC	GATCTGGGTCGCATTCTTGT	qPCR primers for analysis of AaHK1 expression

^a For primer set 1–5, the first nucleotide of the start codon in the AaHK1 gene was considered as position + 1